SmartFlow Technologies

Purification of Bacterial Phage by Continuous Diafiltration

Optimization Procedure

The SmartFlow® filter WORKS Purification of bacterial phage by continuous diafiltration optimization procedure from SFT is intended to purify phage from heavier more viscous cell cultures where complete autolysis has occurred and/or where the phage is dimensionally larger such as 0.08 micron.

This optimization procedure uses a microfiltration (MF) membrane that retain the cells and cell debris and allow the bacterial phage to pass freely through the membrane. The passage characteristics of bacterial phages changes with different buffers, temperatures, concentrations, and membranes. By examining the passage characteristics of the different MF membranes available in the appropriate process conditions, a well defined and executed process development study can identify the most efficient membrane and process conditions to achieve the required performance.