Applications

SmartFlow Technologies has been providing its services to the biopharmaceutical markets since the 1990’s. In that time we have developed the philosophy that we are solving customer problems, providing solutions, and not just selling a product. We work with you to understand your problems and provide thorough applications development and optimization services. These programs provide experienced and dedicated application specialists, process engineers, and validation support engineers to assist you with process development and process optimization programs. From filter selection through complete system design and commissioning, we work with you so that you can quickly realize the benefits provided by SmartFlow® TFF.

Concentration/Diafiltration - SmartFlow's application development has developed hundreds of concentration/diafiltration processes over the years. Key biological/biopharmaceutical protocols are featured in our WORKS program below.
For large scale operations, SmartFlow has developed it's patented DiaSync process. DiaSync enables you to concentrate cellular products from fermentation applications at recycle the product-free media back to the fermentor saving enormous quantities of media and buffers.

Harvesting - Product harvest is a key downstream process. SmartFlow has developed optimized protocols and process development guidelines for cellular, lysed cell, and excreted product harvesting processes detailed in our WORKS program.

Lysate Processing - SmartFlow Technologies has worked with many firms on optimizing e. Coli lysate processing.

Whole Cells - SmartFlow Technologies has developed an optimized process for the concentration and preparation of whole cells from a fermentation broth. Excellent examples include preparing the cells for disruption, storage, or shipping. This procedure uses ultrafiltration membranes that retain the cells and allow the fermentation broth components to pass freely through the membrane. The process can often be run at high flux rates by using a high MW UF membrane that retains the cells but still permits the free passage of the small broth components.

The passage characteristics of broth components change with different buffers, temperatures, concentrations,
and membranes. By examining the passage characteristics of the different UF membranes available in the appropriate process conditions, a well defined and executed process development study can identify the most efficient membrane and process conditions to achieve the required performance.

Applications - WORKS

Concentration and Diafiltration of viral antigens
Concentration and Diafiltration of Whole Bacterial Cells
E Coli lysate by simultaneous process
Inclusion Bodies
Isolation and concentration of small molecules
Isolation of bacterial phage
Isolation of proteins from cell lysate
Isolation of secreted protein by continuous cell diafiltration
Isolation of secreted proteins from mammalian cell culture
Purification of bacterial phage by continuous diafiltration
Purification of Secreted Viral Antigens from cell culture
Purification of viral antigens by continuous diafiltration
Separation of secreted proteins from whole cells bacteria and yeast
Simultaneous isolation and concentration methodology
Ultrafiltration concentration and diafiltration